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簡要描述:BAFF-R Elisa試劑盒可檢測血清、血漿、細胞培養(yǎng)上清或其它相關(guān)生物液體中的含量或者活性表達。進口試劑盒貨期短,物流安全迅速,檢測數(shù)據(jù)準確,是您實驗*,免費為客戶提供全面技術(shù)咨詢服務(wù)及相關(guān)產(chǎn)品中英文雙版說明書,技術(shù)服務(wù):專業(yè),及時,耐心。
產(chǎn)品型號: 96T
所屬分類:ELISA試劑盒進口
更新時間:2024-12-28
BAFF-R Elisa試劑盒是一種敏感性高,特異性強,重復性好的實驗診斷方法,由于其試劑穩(wěn)定、易保存,操作簡便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學檢。
靈敏度,檢測范圍,特異性和重復性:
靈敏度:zui小可測 0.094ng/mL。
檢測范圍:0.156–10ng/mL。
特異性:可檢測重組或天然的待測物質(zhì),且與其它相關(guān)蛋白無交叉反應(yīng)。
重復性:板內(nèi),板間變異系數(shù)均<10%。
BAFF-R ELISA分析檢測試劑盒,英文名稱:BAFF-R ELISA Kit,常用網(wǎng)絡(luò)搜索名:BAFF-R ELISA試劑盒,規(guī)格:48T/96T。
BAFF-R Elisa試劑盒間接法測抗體流程示意圖:
BAFF-R Elisa試劑盒樣本稀釋原則:
首先通過文獻檢索的方式了解待測樣本的大致含量,確定適當?shù)南♂尡稊?shù)。只有稀釋至標準曲線的范圍內(nèi),檢測的結(jié)果才是準確的。稀釋的過程中,應(yīng)做好詳細的記錄。zui后計算濃度時,稀釋了“N”倍,標本的濃度應(yīng)再乘以“N”。
(1)、標準品的稀釋原則:2瓶,每瓶臨用前以樣品稀釋液稀釋至1ml,蓋好后靜置10分鐘以上,然后反復顛倒/搓動以助溶解,其濃度為300 pg/ml,做系列倍比稀釋后,分別稀釋300 pg/ml,150 pg/ml,75 pg/ml,37.5 pg/ml,18.5 pg/ml,9 pg/ml,4.5 pg/ml,樣品稀釋液直接作為標準濃度0 pg/ml,臨用前15分鐘內(nèi)配制。
如配制150 pg/ml標準品:取0.5ml(不要少于0.5ml)300 pg/ml的上述標準品加入含0.5ml樣品稀釋液的Eppendorf管中,混勻即可,其余濃度以此類推。
(2)、BAFF-R Elisa試劑盒*標記抗體的稀釋原則:臨用前以*標記抗體稀釋液稀釋,稀釋前根據(jù)預先計算好的每次實驗所需的總量配制(每孔100μl),實際配制時應(yīng)多配制0.1-0.2ml。如10μl*標記抗體加990μl*標記抗體稀釋液的比例配制,輕輕混勻,在使用前一小時內(nèi)配制。
(3)、辣根過氧化物酶標記親和素的稀釋原則:臨用前以辣根過氧化物酶標記親和素稀釋液稀釋,稀釋前根據(jù)預先計算好的每次實驗所需的總量配制(每孔100μl),實際配制時應(yīng)多配制0.1-0.2ml。如10μl辣根過氧化物酶標記親和素加990μl辣根過氧化物酶標記親和素稀釋液 的比例配制,輕輕混勻,在使用前一小時內(nèi)配制。
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